Effect of dietary macronutrients on intestinal cholesterol absorption and endogenous cholesterol synthesis: a randomized crossover trial

Background and aimsExtensive research showed a diurnal rhythm of endogenous cholesterol synthesis, whereas recent reported no intestinal absorption in males who consumed low-fat meals. Little is known about the acute effect macronutrient consumption on metabolism, hence if meal composition may explain this absence rhythmicity absorption. Therefore, we examined high-fat, high-carbohydrate, high-protein postprandial synthesis apparently healthy overweight slightly obese males.Methods resultsEighteen random order an isoenergetic three occasions. Serum total concentrations, markers (campesterol, cholestanol, sitosterol), intermediates (7-dehydrocholesterol, 7-dehydrodesmosterol, desmosterol, dihydrolanosterol, lanosterol, lathosterol, zymostenol, zymosterol) were measured at baseline (T0) 240 min postprandially (T240). Meal did not significantly change concentrations marker levels (all p > 0.05). 7-dehydrocholesterol, zymosterol decreased between T0 T240 < These decreases different meals 0.05), except for larger decrease dihydrolanosterol after high-fat versus high-carbohydrate (p = 0.009).ConclusionThe influence Several postprandially, but individual macronutrients differentially affect these intermediates, possible dihydrolanosterol.Trial registrationClinicalTrials.gov, NCT03139890.